Application
Immunofluorescence staining of methanol-fixed HeLa cells using Smad2 (Ab-467).Application
Western blot analysis of lysed extracts from HeLa cells using Smad2 (Ab-467).| Product Name | Smad2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.1) Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15 (2): 420–434. Rebecca A. Randall et al. (2004) Mol Cell Biol. February; 24 (3): 1106–1121. Kimberly A Brown et al. (2004) Breast Cancer Res. ; 6 (3): R215–R231. |
| Immunogen | Smad2 (Ab-467) antibody was raised against a peptide sequence around aa. 463~467 (C-S-S-M-S) derived from Human Smad2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD2 |
|---|---|
| Alternative Names | JV18 MADH2 MADR2 JV18-1 hMAD-2 hSMAD2 Mad-related protein 2 |
| Molecular Weight(MW) | 60 kDa |
| Entrez Gene | 4087 |
|---|---|
| Protein Accession | NP_001003652.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Smad2 (Ab-467).
Application
Western blot analysis of lysed extracts from HeLa cells using Smad2 (Ab-467).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
