Application
Western blot analysis of extracts from 293 cells treated with PMA using Phospho-Smad2 (Ser250) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Smad2 (phospho Ser250) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.1) Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagué J (1999)Genes Dev 13, 804-16 . |
| Immunogen | Smad2 (phospho Ser250) antibody was raised against a peptide sequence around phosphorylation site of serine250 (E-L-S (p)-P-T) derived from Human Smad2 . |
| Modification | p-Ser250 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD2 |
|---|---|
| Alternative Names | JV18-1 MADH2 Mad-related protein 2 Mothers against DPP homolog 2 |
| Molecular Weight(MW) | 65 kDa |
| Entrez Gene | 4087 |
|---|---|
| Protein Accession | NP_001003652.1 |
Application
Western blot analysis of extracts from 293 cells treated with PMA using Phospho-Smad2 (Ser250) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
