Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in WB on transfected HEK-293 at: 1:1000 (Lane1: 2ug HEK293lysate, Lane2: 2ug SMAD2 transfected HEK293 lysate).| Product Name | SMAD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.1) Zheng, X., (2006) EMBO J. 25 (3), 615-627. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SMAD2 antibody can be used for detection of SMAD2 by ELISA at 1:1562500. SMAD2 antibody can be used for detection of SMAD2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMAD2 |
|---|---|
| Alternative Names | SMAD2 JV18 MADH2 MADR2 JV18-1 hMAD-2 hSMAD2 |
| Molecular Weight(MW) | 51 kDa, 52 kDa, 48 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in WB on transfected HEK-293 at: 1:1000 (Lane1: 2ug HEK293lysate, Lane2: 2ug SMAD2 transfected HEK293 lysate).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SMAD2 antibody can be used for detection of SMAD2 by ELISA at 1:1562500. SMAD2 antibody can be used for detection of SMAD2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
