Application
Antibody used in WB on Human Jurkat 0.5-1.0 ug/ml.Application
Antibody used in IHC on Human Heart.Application
Antibody used in IHC on Human Lung.| Product Name | SMAD6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.1) Tsunobuchi, H., et al., (2004) Biophys. Res. Commun. 316(3), 712-719. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SMAD6 antibody can be used for detection of SMAD6 by ELISA at 1:62500. SMAD6 antibody can be used for detection of SMAD6 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | SMAD6 |
|---|---|
| Alternative Names | SMAD6 AOVD2 MADH6 MADH7 HsT17432 |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Human Jurkat 0.5-1.0 ug/ml.
Application
Antibody used in IHC on Human Heart.
Application
Antibody used in IHC on Human Lung.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SMAD6 antibody can be used for detection of SMAD6 by ELISA at 1:62500. SMAD6 antibody can be used for detection of SMAD6 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL. |
