Application
Immunohistochemistry staining of SMAD6 in testis tissue using SMAD6 Antibody.| Product Name | SMAD6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used asalternate namesfor one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1. |
| Immunogen | SMAD6 antibody was raised against amino acids 85 - 99 of SMAD6 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| SMAD6 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 3 μg/mL. | |
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SMAD6 |
|---|---|
| Alternative Names | SMAD6 AOVD2 HSMAD6 HsT17432 SMAD 6 MADH7 Mothers against DPP homolog 6 SMAD family member 6 MAD homolog 6 MADH6 |
Application
Immunohistochemistry staining of SMAD6 in testis tissue using SMAD6 Antibody.| Application Notes | SMAD6 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 3 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SMAD6 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
