Application
Antibody used in CHIP on HCT116 Cells.Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | SMARCA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMARCA5 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA5 is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II.The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMARCA5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SMARCA5 antibody can be used for detection of SMARCA5 by ELISA at 1:312500. SMARCA5 antibody can be used for detection of SMARCA5 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMARCA5 |
|---|---|
| Alternative Names | SMARCA5 ISWI SNF2H WCRF135 hISWI hSNF2H |
| Molecular Weight(MW) | 122 kDa |
Application
Antibody used in CHIP on HCT116 Cells.
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | SMARCA5 antibody can be used for detection of SMARCA5 by ELISA at 1:312500. SMARCA5 antibody can be used for detection of SMARCA5 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMARCA5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
