Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1 (Phospho-Ser957).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using SMC1 (Phospho-Ser957).| Product Name | SMC1 (phospho Ser957) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.1) Yang XM. et al. (2004) Mol Cell Biol. 24 (12): 5290-5303. Kim ST. et al. (2002) Genes Dev. 16 (5): 560-570. Revenkova E. et al. (2001) Mol Cell Biol. 21 (20): 6984-6998 |
| Immunogen | SMC1 (Phospho-Ser957) antibody was raised against a peptide sequence around phosphorylation site of serine 957 (G-S-S (p) -Q-G) derived from Human SMC1. |
| Modification | p-Ser957 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMC1A |
|---|---|
| Alternative Names | SMC1 SMCB CDLS2 SB1.8 SMC1L1 DXS423E SMC1alpha APRF SM1A SMC1A SMC1 alpha protein |
| Molecular Weight(MW) | 145 kDa |
| Entrez Gene | 8243 |
|---|---|
| Protein Accession | NP_006297.2 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1 (Phospho-Ser957).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using SMC1 (Phospho-Ser957).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
