Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | SMC1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq].1) Limongelli, G., et al. Am. J. Med. Genet.152A (8), 2127-2129 (2010) : |
| Immunogen | This SMC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-336 amino acids from the N-terminal region of human SMC1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMC1A |
|---|---|
| Alternative Names | Structural maintenance of chromosomes protein 1A SMC protein 1A SMC-1-alpha SMC-1A Sb18 SMC1A DXS423E KIAA0178 SB18 SMC1 SMC1L1 |
| Molecular Weight(MW) | 143 kDa |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
