Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of SMYD3 antibody in CEM cell line lysates (35ug/lane)Application
Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody at 1:1000.| Product Name | SMYD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.1) Zou,J.N., Wang,S.Z. Cancer Lett. 280 (1), 78-85 (2009) |
| Immunogen | This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SMYD3 |
|---|---|
| Alternative Names | Histone-lysine N-methyltransferase SMYD3 SET and MYND domain-containing protein 3 Zinc finger MYND domain-containing protein 1 SMYD3 ZMYND1 ZNFN3A1 |
| Molecular Weight(MW) | 49 kDa |
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of SMYD3 antibody in CEM cell line lysates (35ug/lane)
Application
Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody at 1:1000.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
