Application
Immunofluorescence staining of methanol-fixed HuvEc cells using SNAI1 (Phospho-Ser246) Antibody.Application
Western blot analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | SNAI1 (phospho Ser246) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.1) Okubo T., Cancer Res. 61:1338-1346(2001). |
| Immunogen | SNAI1 (phospho Ser246) antibody was raised against a peptide sequence around phosphorylation site of Serine 246 (T-F-S (p)-R-M) derived from Human SNAI1. |
| Modification | p-Ser246 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNAI1 |
|---|---|
| Alternative Names | SNAH SNAI Sna Snail |
| Molecular Weight(MW) | 29 kDa |
| Entrez Gene | 6615 |
|---|---|
| Protein Accession | NP_005976.2 |
Application
Immunofluorescence staining of methanol-fixed HuvEc cells using SNAI1 (Phospho-Ser246) Antibody.
Application
Western blot analysis of extracts from HT29 cells using SNAI1 (Phospho-Ser246) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
