Application
Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | SNAP25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.1) Shimada, M., et al. Hum. Genet. 128(4):433-441(2010) |
| Immunogen | This SNAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the Central region of human SNAP25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNAP25 |
|---|---|
| Alternative Names | Synaptosomal-associated protein 25 SNAP-25 Super protein SUP Synaptosomal-associated 25 kDa protein SNAP25 SNAP |
| Molecular Weight(MW) | 23 kDa |
Application
Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
