Application
Antibody used in WB on Human NT-2 and Mouse WT brain at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate Lane 2. Mouse WT brain extract (80ug)).Application
Antibody used in WB on Human Frontal Cortex at 0.2-1 ug/ml.| Product Name | SNAP25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Snap25 contains 2 t-SNARE coiled-coil homology domains and belongs to the SNAP-25 family. t-SNARE involved in the molecular regulation of neurotransmitter release. Snap25 may play an important role in the synaptic function of specific neuronal systems. Snap25 associates with proteins involved in vesicle docking and membrane fusion.1) Nagy, G., et al., (2005) Mol. Biol. Cell 16 (12), 5675-5685. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SNAP25. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SNAP25 antibody can be used for detection of SNAP25 by ELISA at 1:312500. SNAP25 antibody can be used for detection of SNAP25 by western blot at 1.0μ μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNAP25 |
|---|---|
| Alternative Names | SNAP25 sp Bdr SNAP-25 |
| Molecular Weight(MW) | 23 kDa |
Application
Antibody used in WB on Human NT-2 and Mouse WT brain at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate Lane 2. Mouse WT brain extract (80ug)).
Application
Antibody used in WB on Human Frontal Cortex at 0.2-1 ug/ml.| Positive Control | 1) Frontal Cortex Lysate |
|---|---|
| Application Notes | SNAP25 antibody can be used for detection of SNAP25 by ELISA at 1:312500. SNAP25 antibody can be used for detection of SNAP25 by western blot at 1.0μ μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SNAP25 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
