SNRPA binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.1) Shajani, Z. (2005) J. Mol. Biol. 349 (4), 699-715.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SNRPA.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
SNRPA antibody can be used for detection of SNRPA by ELISA at 1:312500. SNRPA antibody can be used for detection of SNRPA by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Prostate at 5.0 ug/ml.
Positive Control
1) 293T Cell Lysate
Application Notes
SNRPA antibody can be used for detection of SNRPA by ELISA at 1:312500. SNRPA antibody can be used for detection of SNRPA by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SNRPA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.