Application
Antibody used in IHC on Human kidney.Application
Antibody used in WB on Human cell lines at: 1:500 (Lane 1: 50ug HeLa lysate, Lane 2: 50ug 293T lysate, Lane 3: 50ug K562 lysate, Lane 4: 50ug MDA-MB-231 lysate).Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Product Name | SOD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in its gene have been implicated as causes of familial amyotrophic lateral sclerosis.The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.1) Hays, A.P., J. Neurol. Sci. 242 (1-2), 67-69 (2006). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOD1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SOD1 antibody can be used for detection of SOD1 by ELISA at 1:312500. SOD1 antibody can be used for detection of SOD1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SOD1 |
|---|---|
| Alternative Names | SOD1 ALS ALS1 IPOA SOD homodimer hSod1 HEL-S-44 |
| Molecular Weight(MW) | 16 kDa |
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in WB on Human cell lines at: 1:500 (Lane 1: 50ug HeLa lysate, Lane 2: 50ug 293T lysate, Lane 3: 50ug K562 lysate, Lane 4: 50ug MDA-MB-231 lysate).
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SOD1 antibody can be used for detection of SOD1 by ELISA at 1:312500. SOD1 antibody can be used for detection of SOD1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SOD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
