Application
Western blot analysis of anti-SOD2 Pab in K562 cell line lysates (35ug/lane)Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SOD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.1) Fabre,E.E., Am. J. Clin. Nutr. 87 (5), 1504-1512 (2008) Kaewpila,S., Cancer Res. 68 (8), 2781-2788 (2008) Flekac,M., (er) BMC Med. Genet. 9, 30 (2008) |
| Immunogen | This SOD2 antibody is generated from rabbits immunized with human recombinant SD protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SOD2 |
|---|---|
| Alternative Names | Superoxide dismutase [Mn] mitochondrial SOD2 |
| Molecular Weight(MW) | 25 kDa |
Application
Western blot analysis of anti-SOD2 Pab in K562 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
