SOX18 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.1) Spataro, V., et al., (2005) Arterioscler. Thromb. Vasc. Biol. 25 (11), 2398-2403.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOX18.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
SOX18 antibody can be used for detection of SOX18 by ELISA at 1:62500. SOX18 antibody can be used for detection of SOX18 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human heart at 0.2-1 ug/ml.
Application
Antibody used in IF on Human lymphatic endothelial at 1:50 -1:500.
Application
Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.
Positive Control
1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Application Notes
SOX18 antibody can be used for detection of SOX18 by ELISA at 1:62500. SOX18 antibody can be used for detection of SOX18 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SOX18 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.