Application
Immunofluorescence of SOX4 in rat testis tissue with SOX4 antibody at 20 μg/mL.Application
Immunohistochemistry of SOX4 in rat testis tissue with SOX4 antibody at 5 μg/mL.Application
Western blot analysis of SOX4 in human testis tissue lysate with SOX4 antibody at 1 μg/ml.| Product Name | SOX4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRY (Sex determining region Y)-box 4, Transcription factor SOX-4, EVI16 |
| Product description | SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate (1,2). It may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin) (3). SOX4 contains one HMG box DNA-binding domain and may function in the apoptosis pathway leading to cell death. It has also been suggested to play a role in tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development (4,5).1) Wotton D, Lake RA, Farr CJ, et al. The high mobility group transcription factor, SOX4, transactivates the human CD2 enhancer. J. Biol. Chem. 1995; 270:7515-22. |
| Immunogen | SOX4 antibody was raised against a 16 amino acid peptide near the amino terminus of human SOX4. The immunogen is located within the first 50 amino acids of SOX4. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 IF:1:50~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOX4 |
|---|---|
| Alternative Names | SRY (Sex determining region Y)-box 4 Transcription factor SOX-4 EVI16 |
| Molecular Weight(MW) | Predicted: 52 kDa Observed: 520 kDa |
| Tissue Specificity | SOX4 antibody is human, mouse and rat reactive. |
Application
Immunofluorescence of SOX4 in rat testis tissue with SOX4 antibody at 20 μg/mL.
Application
Immunohistochemistry of SOX4 in rat testis tissue with SOX4 antibody at 5 μg/mL.
Application
Western blot analysis of SOX4 in human testis tissue lysate with SOX4 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 IF:1:50~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | SOX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SOX4 antibody is supplied in PBS containing 0.02% sodium azide. |
