Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Product Name | SOX9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.1) Furumatsu, T., et al., (2005) J. Biol. Chem. 280 (42), 35203-35208. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOX9. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SOX9 antibody can be used for detection of SOX9 by ELISA at 1:12500. SOX9 antibody can be used for detection of SOX9 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SOX9 |
|---|---|
| Alternative Names | SOX9 CMD1 SRA1 CMPD1 |
| Molecular Weight(MW) | 56 kDa |
Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SOX9 antibody can be used for detection of SOX9 by ELISA at 1:12500. SOX9 antibody can be used for detection of SOX9 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SOX9 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
