Application Antibody used in WB on Human U2OS at 1:1000.
Application Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Application Antibody used in IHC on Human Prostate at 5.0 ug/ml.
| Product Name | SP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SP3 is a transcriptional factor that can act as an activator or repressor, probably in a isoform-specific manner. SP3 binds to GT and GC boxes promoters elements.This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted.1) Whitten, C., Gene 413 (1-2), 58-66 (2008). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SP3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| SP3 antibody can be used for detection of SP3 by ELISA at 1:312500. SP3 antibody can be used for detection of SP3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SP3 |
|---|---|
| Alternative Names | SP3 DKFZp686O1631 SPR-2 SPR2 |
| Molecular Weight(MW) | 82 kDa |
Application Antibody used in WB on Human U2OS at 1:1000.
Application Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Application Antibody used in IHC on Human Prostate at 5.0 ug/ml.
| Positive Control | 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | SP3 antibody can be used for detection of SP3 by ELISA at 1:312500. SP3 antibody can be used for detection of SP3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SP3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
