Application
Western blot analysis of SP5 Antibody in mouse liver tissue lysates (35ug/lane)Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SP5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription factor Sp5, SP5 |
| Product description | SP5 binds to GC boxes promoters elements. SP5 probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo.1) Chen, Y., et al. Biochem. Biophys. Res. Commun. 340(3):758-766(2006) |
| Immunogen | This SP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-398 amino acids from the C-terminal region of human SP5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | SP5 |
|---|---|
| Alternative Names | Transcription factor Sp5 SP5 |
| Molecular Weight(MW) | 42 kDa |
Application
Western blot analysis of SP5 Antibody in mouse liver tissue lysates (35ug/lane)
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
