Application
Antibody used in WB on Human MCF7 cells at 2.5 ug/ml.| Product Name | SPDEF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPDEF, PDEF, RP11-375E1__A.3, bA375E1.3 |
| Product description | PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA expression.PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.1) Fowler, M., (2006) J. Cell. Biochem. 97 (1), 1-17. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPDEF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SPDEF antibody can be used for detection of SPDEF by ELISA at 1:12500. SPDEF antibody can be used for detection of SPDEF by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPDEF |
|---|---|
| Alternative Names | SPDEF PDEF RP11-375E1__A.3 bA375E1.3 |
| Molecular Weight(MW) | 38 kDa |
Application
Antibody used in WB on Human MCF7 cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | SPDEF antibody can be used for detection of SPDEF by ELISA at 1:12500. SPDEF antibody can be used for detection of SPDEF by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPDEF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
