Application
Western blot analysis of SPDYA using rabbit polyclonal SPDYA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPDYA gene.Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
SPDYA Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | SPDYA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Speedy protein A, Rapid inducer of G2/M progression in oocytes A, RINGO A, hSpy/Ringo A, Speedy-1, Spy1, SPDYA ( HGNC:30613 ) |
| Product description | SPDYA (speedy homolog A (Drosophila)) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. SPDYA can activate CDK2 without promoting CDK2 phosphorylation. SPDYA mediates cell survival during the DNA damage process through activation of CDK2.1) Ke, Q., et al. Exp. Mol. Pathol. 87(3):167-172(2009) |
| Immunogen | This SPDYA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the Central region of human SPDYA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPDYA |
|---|---|
| Alternative Names | Speedy protein A Rapid inducer of G2/M progression in oocytes A RINGO A hSpy/Ringo A Speedy-1 Spy1 SPDYA ( HGNC:30613 ) |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of SPDYA using rabbit polyclonal SPDYA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPDYA gene.
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
SPDYA Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
