Application
Immunohistochemistry staining of SPHK1 in adrenal, zona glomerulosa tissue using SPHK1 Antibody.| Product Name | SPHK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPHK1, SK1, Sphingosine kinase 1, SPHK, SK 1, SPK 1, Sphingosine kinase type 1, SPK |
| Product description | Sphingosine kinase (SPHK) phosphorylates sphingosine to form a bioactive lipid mediate, sphingosine 1-phosphate (S1P or SPP). S1P is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival while extracellularly it is a ligand for EDG 1. Expression of SPHK1 and subsequent production of S1P promotes the G1-S transition, protects cells from apoptosis, stimulates migration and angiogenesis, and induces tumor formation in mice. |
| Immunogen | SPHK1 antibody was raised against a peptide located in the N-Terminal domain of SPHK1 (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC |
| SPHK1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
|
| Species Reactivity | GorillaHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPHK1 |
|---|---|
| Alternative Names | SPHK1 SK1 Sphingosine kinase 1 SPHK SK 1 SPK 1 Sphingosine kinase type 1 SPK |
| Tissue Specificity | BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Application
Immunohistochemistry staining of SPHK1 in adrenal, zona glomerulosa tissue using SPHK1 Antibody.| Application Notes | SPHK1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | SPHK1 antibody should be stored long term (months) at -80 ˚C and short term (days) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.1% sodium azide. |
