Application
Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/mL.Application
Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/ml.Application
Western blot analysis of SPIB in human prostate tissue lysate with SPIB antibody at 1 μg/ml.| Product Name | SPIB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Spi-B transcription factor, SPI-B |
| Product description | SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1). SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3). It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN)-producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4).1) Ray D, Bosselut R, Ghysdael J, et al. Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1. Mol. Cell Biol. 1992; 12:4297-304. |
| Immunogen | SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB. The immunogen is located within amino acids 200 - 250 of SPIB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPIB |
|---|---|
| Alternative Names | Spi-B transcription factor SPI-B |
| Molecular Weight(MW) | Predicted: 19, 25, 29 kDa Observed: 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (88%) |
| Tissue Specificity | SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3. |
Application
Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/mL.
Application
Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/ml.
Application
Western blot analysis of SPIB in human prostate tissue lysate with SPIB antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1312 - Human Prostate Tissue Lysate |
|---|---|
| Application Notes | SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPIB antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SPIB antibody is supplied in PBS containing 0.02% sodium azide. |
