Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SQLE.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
SQLE antibody can be used for detection of SQLE by ELISA at 1:312500. SQLE antibody can be used for detection of SQLE by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human 721_B cells at 0.25 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
SQLE antibody can be used for detection of SQLE by ELISA at 1:312500. SQLE antibody can be used for detection of SQLE by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SQLE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.