Application
Western blot analysis of extracts from COLO205 tissue using Src (Phospho-Ser75) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Src (phospho Ser75) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p60-Src, c-Src, pp60c-src |
| Product description | proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.1) Pan Q, et al. (2011)Cell Mol Life Sci 68, 3425-36 . Kato G, Maeda S (2003) J Biochem (Tokyo) 133, 563-9. Kato G, Maeda S (1999)J Biochem (Tokyo) 126, 957-61. |
| Immunogen | Src (phospho Ser75) antibody was raised against a peptide sequence around phosphorylation site of serine 75 (V-T-S (p)-P-Q) derived from Human Src. |
| Modification | p-Ser75 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRC |
|---|---|
| Alternative Names | p60-Src c-Src pp60c-src |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | The antibody detects endogenous level of Src only when phosphorylated at serine 75. |
| Entrez Gene | 6714 |
|---|---|
| Protein Accession | NP_005408.1 |
Application
Western blot analysis of extracts from COLO205 tissue using Src (Phospho-Ser75) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
