Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Src (Phospho-Tyr529).Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).| Product Name | Src (phospho Tyr529) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASV, SRC1, c-SRC, p60-Src, PKB, RAC, C-SRC |
| Product description | proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.1) Pyper J. M., (1985) Mol. Cell. Biol. 5:831-838Pyper J. M. (1990) Mol. Cell. Biol. 10:2035-2040Xu W., (1997) . Nature 385:595-602Benes C. H., (2005) Cell 121:271-280 |
| Immunogen | Src (Phospho-Tyr529) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y (p) -Q-P) derived from Human Src. |
| Modification | p-Tyr529 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRC |
|---|---|
| Alternative Names | ASV SRC1 c-SRC p60-Src PKB RAC C-SRC |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of Src only when phosphorylated at tyrosine 529. |
| Entrez Gene | 6714 |
|---|---|
| Protein Accession | NP_005408.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Src (Phospho-Tyr529).
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
