Application
Antibody used in WB on mouse glomerular endothelial at 1:1000 (Lane 1: 50ug mouse glomerular endothelial lysate Lane 2: 50ug mouse glomerular endothelial lysate ).Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.| Product Name | SREBF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SREBF1, ADD1, ADD-1, SREBP1, bHLHd1, SREBP-1, SREBP1c, SREBP-1a, SREBP-1c, D630008H06 |
| Product description | Srebf1 is transcriptional activator that binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Srebf1 also binds to an E-box motif (5'-ATCACGTGA-3'). Srebf1 regulates the transcription of genes for sterol biosynthesis and the LDL receptor gene.1) Briancon, N. (2006) EMBO J. 25 (6), 1253-1262. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SREBF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SREBF1 antibody can be used for detection of SREBF1 by ELISA at 1:62500. SREBF1 antibody can be used for detection of SREBF1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF1 |
|---|---|
| Alternative Names | SREBF1 ADD1 ADD-1 SREBP1 bHLHd1 SREBP-1 SREBP1c SREBP-1a SREBP-1c D630008H06 |
| Molecular Weight(MW) | 125 kDa |
Application
Antibody used in WB on mouse glomerular endothelial at 1:1000 (Lane 1: 50ug mouse glomerular endothelial lysate Lane 2: 50ug mouse glomerular endothelial lysate ).
Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.| Positive Control | 1) SP2/0 Cell Lysate |
|---|---|
| Application Notes | SREBF1 antibody can be used for detection of SREBF1 by ELISA at 1:62500. SREBF1 antibody can be used for detection of SREBF1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SREBF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
