Application
Antibody used in IHC on Human Liver.Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.Application
Antibody used in IHC.| Product Name | SREBF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SREBF1, SREBP1, bHLHd1, SREBP-1c |
| Product description | SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.1) Motoyama, K., (2006) Biochem. Biophys. Res. Commun. 342 (1), 354-360. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SREBF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SREBF1 antibody can be used for detection of SREBF1 by ELISA at 1:62500. SREBF1 antibody can be used for detection of SREBF1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF1 |
|---|---|
| Alternative Names | SREBF1 SREBP1 bHLHd1 SREBP-1c |
| Molecular Weight(MW) | 125 kDa |
| Entrez Gene | 6720 |
|---|---|
| Protein Accession | NP_001005291 |
Application
Antibody used in IHC on Human Liver.
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Application
Antibody used in IHC.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | SREBF1 antibody can be used for detection of SREBF1 by ELISA at 1:62500. SREBF1 antibody can be used for detection of SREBF1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SREBF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
