Application
Immunohistochemistry of SREBF1 in human spleen tissue with SREBF1 antibody at 5 μg/mL.Application
Immunofluorescence of SREBF1 in human spleen tissue with SREBF1 antibody at 20 μg/mL.Application
Western blot analysis of SREBF1 in Daudi cell lysate with SREBF1 antibody at 1 μg/ml.| Product Name | SREBF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SREBF1 Antibody: SREBP1, bHLHd1, SREBP-1c, BHLHD1, SREBP1, Sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, SREBP-1 |
| Product description | The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1). The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2). SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3). The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4).1) Wang X, Briggs MR, Hua X, et al. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. II. Purification and characterization. J. Biol. Chem. 1993; 268:14497-504. |
| Immunogen | SREBF1 antibody was raised against a 17 amino acid peptide near the center of human SREBF1. The immunogen is located within amino acids 450 - 500 of SREBF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF1 |
|---|---|
| Alternative Names | SREBF1 Antibody: SREBP1 bHLHd1 SREBP-1c BHLHD1 SREBP1 Sterol regulatory element-binding protein 1 Class D basic helix-loop-helix protein 1 SREBP-1 |
| Molecular Weight(MW) | Predicted: 129 kDa Observed: 132 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | SREBF1 antibody is human and mouse reactive. At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2. |
| Entrez Gene | 6720 |
|---|---|
| Protein Accession | NP_001005291 |
Application
Immunohistochemistry of SREBF1 in human spleen tissue with SREBF1 antibody at 5 μg/mL.
Application
Immunofluorescence of SREBF1 in human spleen tissue with SREBF1 antibody at 20 μg/mL.
Application
Western blot analysis of SREBF1 in Daudi cell lysate with SREBF1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SREBF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SREBF1 antibody is supplied in PBS containing 0.02% sodium azide. |
