Application
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | SRP14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Signal recognition particle 14 kDa protein, SRP14, 18 kDa Alu RNA-binding protein, SRP14 |
| Product description | Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.1) Mary, C., et al. RNA 16(5):969-979(2010) |
| Immunogen | This SRP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-55 amino acids from the Central region of human SRP14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRP14 |
|---|---|
| Alternative Names | Signal recognition particle 14 kDa protein SRP14 18 kDa Alu RNA-binding protein SRP14 |
| Molecular Weight(MW) | 15 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
