Application
Immunohistochemistry of SRPK1 in human testis tissue with SRPK1 antibody at 5 μg/ml.Application
Western blot analysis of SRPK1 in mouse heart tissue lysate with SRPK1 antibody at 1 μg/ml.| Product Name | SRPK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRSF protein kinase 1, Serine/arginine-rich protein-specific kinase 1, Serine/threonine-protein kinase SRPK1, SFRSK1, SR-protein-specific kinase 1 |
| Product description | SRPK1 and SRPK2, two Arginine/serine-rich (SR) protein-specific kinases constitute control circuits to regulate both constitutive and alternative pre-mRNA splicing and coordinate splicing with transcription in cells (1). They are highly specific for the phosphorylation of these RS proteins, and highly expressed in testis (1,2). SRPK1 plays a role in the condensation of sperm chromatin and controls the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis (3,4).1) Wang HY, Arden KC, Bermingham JR Jr, et al. Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. Genomics 1999; 57:310-5. |
| Immunogen | SRPK1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human SRPK1. The immunogen is located within the first 50 amino acids of SRPK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| SRPK1 antibody can be used for the detection of SRPK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 5 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRPK1 |
|---|---|
| Alternative Names | SRSF protein kinase 1 Serine/arginine-rich protein-specific kinase 1 Serine/threonine-protein kinase SRPK1 SFRSK1 SR-protein-specific kinase 1 |
| Molecular Weight(MW) | Predicted: 54, 72, 91 kDa Observed: 91 kDa |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2. |
Application
Immunohistochemistry of SRPK1 in human testis tissue with SRPK1 antibody at 5 μg/ml.
Application
Western blot analysis of SRPK1 in mouse heart tissue lysate with SRPK1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | SRPK1 antibody can be used for the detection of SRPK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 5 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SRPK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SRPK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
