Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | SSRP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SSRP1,SSRP 1, FACT, FACT80, T160 |
| Product description | SSRP1 is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. SSRP1 contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. It also functions as a co-activator of the transcriptional activator p63.The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Li, Y., (2007) J. Biol. Chem. 282 (10), 6936-6945. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SSRP1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SSRP1 antibody can be used for detection of SSRP1 by ELISA at 1:1562500. SSRP1 antibody can be used for detection of SSRP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SSRP1 |
|---|---|
| Alternative Names | SSRP1 SSRP 1 FACT FACT80 T160 |
| Molecular Weight(MW) | 81 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | SSRP1 antibody can be used for detection of SSRP1 by ELISA at 1:1562500. SSRP1 antibody can be used for detection of SSRP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SSRP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
