Synthesis of alpha-2,3-linked sialic acid to Gal (beta-1,3)GalNAc is mediated by at least 3 distinct beta-galactoside alpha-2,3-sialyltransferases (EC 2.4.99.4), including ST3GAL4. In contrast, only a single gene encodes the beta-galactoside alpha-2,6-sialyltransferase, ST6GAL1.1) Moody, A.M., (2003) J. Biol. Chem. 278 (9), 7240-7246.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ST3GAL4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
ST3GAL4 antibody can be used for detection of ST3GAL4 by ELISA at 1:312500. ST3GAL4 antibody can be used for detection of ST3GAL4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
ST3GAL4 antibody can be used for detection of ST3GAL4 by ELISA at 1:312500. ST3GAL4 antibody can be used for detection of ST3GAL4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ST3GAL4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.