Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1 (Phospho-Tyr701).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with IFN using STAT1 (Phospho-Tyr701).| Product Name | STAT1 (phospho Tyr701) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CANDF7, ISGF-3, STAT91, BHLHE39, Transcription factor ISGF-3 components p91/p84 |
| Product description | Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-γ-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.1) Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Durbin J E, et al. (1996) Cell. 84: 443-450. Meraz M A, et al. (1996) Cell. 84: 431-442. Wakao H, et al. (1994) EMBO J. 13: 2182-2191. Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258 Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227. |
| Immunogen | STAT1 (Phospho-Tyr701) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y (p) -I-K) derived from Human STAT1. |
| Modification | p-Tyr701 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT1 |
|---|---|
| Alternative Names | CANDF7 ISGF-3 STAT91 BHLHE39 Transcription factor ISGF-3 components p91/p84 |
| Molecular Weight(MW) | 91 kDa |
| Tissue Specificity | This antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701. |
| Entrez Gene | 6772 |
|---|---|
| Protein Accession | NP_009330.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1 (Phospho-Tyr701).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with IFN using STAT1 (Phospho-Tyr701).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
