Application
Immunocytochemistry of STAT1 alpha in HeLa cells with STAT1 alpha antibody at 10 μg/mL.Application
Immunohistochemistry of STAT1alpha in human colon tissue with STAT1alpha antibody at 2.5 μg/ml.Application
Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1a antibody at 1 μg/mL.| Product Name | STAT1 alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT1 alpha Antibody: CANDF7, ISGF-3, STAT91 |
| Product description | STAT1 alpha Antibody: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-α, IFN-β, IFN-γ and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1).1) Leonard WJ and O'Shea JJ. Jaks and STATs: biological implications. Annu. Rev. Immunol. 1998; 16:293-322. |
| Immunogen | STAT1 alpha antibody was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The sequences differ from the murine corresponding sequences by four amino acids. The immunogen is located within the last 50 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIHC-PIPWB |
| STAT1 alpha antibody can be used for Western blot at 0.5 - 1 μg and for immunoprecipitation at 2 to 4 μg per sample. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT1 |
|---|---|
| Alternative Names | STAT1 alpha Antibody: CANDF7 ISGF-3 STAT91 |
| Molecular Weight(MW) | Predicted: 91 kDa Observed: 90 kDa |
| Sequence Similarities | 4) Cat. No. 1204 - K562 Cell Lysate |
| Tissue Specificity | At least two isoforms of STAT1 are known to exist; this antibody will only recognize the larger isoform. |
Application
Immunocytochemistry of STAT1 alpha in HeLa cells with STAT1 alpha antibody at 10 μg/mL.
Application
Immunohistochemistry of STAT1alpha in human colon tissue with STAT1alpha antibody at 2.5 μg/ml.
Application
Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1a antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | STAT1 alpha antibody can be used for Western blot at 0.5 - 1 μg and for immunoprecipitation at 2 to 4 μg per sample. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | STAT1 alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide. |
