Application
Antibody used in WB on Recombinant STAT1 protein at 5 ug/ml.| Product Name | STAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT1, DKFZp686B04100, ISGF-3, STAT91, CANDF7 |
| Product description | STAT1 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.Western blots using four different antibodies against three unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.1) Battle, T.E., (2006) Cancer Res. 66 (7), 3649-3657. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human STAT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| STAT1 antibody can be used for detection of STAT1 by ELISA at 1:62500. STAT1 antibody can be used for detection of STAT1 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT1 |
|---|---|
| Alternative Names | STAT1 DKFZp686B04100 ISGF-3 STAT91 CANDF7 |
| Molecular Weight(MW) | 87 kDa |
Application
Antibody used in WB on Recombinant STAT1 protein at 5 ug/ml.| Application Notes | STAT1 antibody can be used for detection of STAT1 by ELISA at 1:62500. STAT1 antibody can be used for detection of STAT1 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Form | Liquid |
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| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store STAT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
