Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Ab-705). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Ab-705) antibody (#21045, Red).Application
Western blot analysis of lysed extracts from HeLa cells using STAT3 (Ab-705).| Product Name | STAT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APRF, HIES, BSP1, MADH1, MADR1, APRF, Acute-phase response factor |
| Product description | Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.1) Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804. Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115. Garcia R, et al. (1998) J Biomed Sci. 5: 79-85. Bromberg J F, et al. (1999) Cell. 98: 295-303. |
| Immunogen | STAT3 (Ab-705) antibody was raised against a peptide sequence around aa.703~707 (A-P-Y-L-K) derived from Human STAT3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: IF:1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT3 |
|---|---|
| Alternative Names | APRF HIES BSP1 MADH1 MADR1 APRF Acute-phase response factor |
| Molecular Weight(MW) | 88 kDa |
| Tissue Specificity | This antibody detects endogenous level of total STAT3 protein. |
| Entrez Gene | 6774 |
|---|---|
| Protein Accession | NP_003141.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Ab-705). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Ab-705) antibody (#21045, Red).
Application
Western blot analysis of lysed extracts from HeLa cells using STAT3 (Ab-705).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: IF:1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
