Application
Western blot analysis of lysed extracts from HeLa and HepG2 cells untreated or treated with PMA using STAT3 (Phospho-Ser727).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Ser727). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Phospho-Ser727).IF
(B) Immunofluorescent of STAT3 and phosphorylated STAT3 at pSTAT3tyr705 and pSTAT3ser727 (40x). Green represents the STAT 3 or its phosphorylation, blue represents the Dapi stain.| Product Name | STAT3 (phospho Ser727) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.1) Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804. Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115. Garcia R, et al. (1998) J Biomed Sci. 5: 79-85. Bromberg J F, et al. (1999) Cell. 98: 295-303. |
| Immunogen | STAT3 (Phospho-Ser727) antibody was raised against a peptide sequence around phosphorylation site of serine 727 (P-M-S (p) -P-R) derived from Human STAT3. |
| Modification | p-Ser727 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT3 |
|---|---|
| Alternative Names | APRF HIES STAT5 APRF Acute-phase response factor |
| Molecular Weight(MW) | 88 kDa |
| Tissue Specificity | This antibody detects endogenous level of STAT3 only when phosphorylated at serine 727. |
| Entrez Gene | 6774 |
|---|---|
| Protein Accession | NP_003141.2 |
Application
Western blot analysis of lysed extracts from HeLa and HepG2 cells untreated or treated with PMA using STAT3 (Phospho-Ser727).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Ser727). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Phospho-Ser727).
IF
(B) Immunofluorescent of STAT3 and phosphorylated STAT3 at pSTAT3tyr705 and pSTAT3ser727 (40x). Green represents the STAT 3 or its phosphorylation, blue represents the Dapi stain.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
