Application
Antibody used in IHC on Mouse at 1:1000.Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Product Name | STAT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT3, APRF, FLJ20882, MGC16063, HIES |
| Product description | STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.1) Ohbayashi, N., (2008) Biochem. Biophys. Res. Commun. 371 (4), 823-828. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human STAT3. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| STAT3 antibody can be used for detection of STAT3 by ELISA at 1:1562500. STAT3 antibody can be used for detection of STAT3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT3 |
|---|---|
| Alternative Names | STAT3 APRF FLJ20882 MGC16063 HIES |
| Molecular Weight(MW) | 88 kDa |
Application
Antibody used in IHC on Mouse at 1:1000.
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | STAT3 antibody can be used for detection of STAT3 by ELISA at 1:1562500. STAT3 antibody can be used for detection of STAT3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store STAT3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
