Application
Western blot analysis of lysed extracts from HepG2 cells using STAT6 (Ab-641).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6 (Ab-641).| Product Name | STAT6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT6B, STAT6C, D12S1644, IL-4-STAT, IL-4 Stat |
| Product description | STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. By screening an embryonic lung fibroblast cDNA library with a wildtype STAT6 probe, 2 variant cDNAs were identified, which were termed STAT6B and STAT6C, encoding an N-terminal 110-amino acid truncation and a 27-amino acid deletion in the SH2 domain, respectively. All the three variants are ubiquitously expressed with STAT6B expression greatest in spleen and STAT6C expression greatest in lung. STAT6B resembles an attenuated STAT6, but that STAT6C inhibits IL-4-mediated mitogenesis and cell surface antigen expression, and is not tyrosine phosphorylated1) Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738. Malabarba M G, et al. (1996) Biochem. J. 319:865-872. Hou J, et al. (1994) Science. 265:1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343. Takeda K, et al. (1996) Nature. 380: 627-630. |
| Immunogen | STAT6 (Ab-641) antibody was raised against a peptide sequence around aa.639~643 (R-G-Y-V-P) derived from Human STAT6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT6 |
|---|---|
| Alternative Names | STAT6B STAT6C D12S1644 IL-4-STAT IL-4 Stat |
| Molecular Weight(MW) | 110 kDa |
| Tissue Specificity | This antibody detects endogenous level of total STAT6 protein. |
| Entrez Gene | 6778 |
|---|---|
| Protein Accession | NP_003144.3 |
Application
Western blot analysis of lysed extracts from HepG2 cells using STAT6 (Ab-641).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6 (Ab-641).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
