Application
Western blot analysis of lysed extracts from 293 and HeLa cells using Stathmin 1 (Ab-25).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1 (Ab-25). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Stathmin 1 (Ab-25).| Product Name | Stathmin 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, CSDD1, LIN28, ZCCHC1, STMN1, STN1, stathmin |
| Product description | Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear1) Boehm M, et al. (2002) EMBO J 21 (13): 3390-3401. Vadlamudi RK, et al. (2005) Mol Cell Biol 25 (9): 3726-3736. Zilfou JT, et al. (2001) Mol Cell Biol 21 (12): 3974-3985. Biernat J, et al. (2002) Mol Biol Cell 13 (11): 4013-4028. |
| Immunogen | Stathmin 1 (Ab-25) antibody was raised against a peptide sequence around aa. 23~27 (I-L-S-P-R) derived from Human Stathmin 1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STMN1 |
|---|---|
| Alternative Names | Lag SMN OP18 PP17 PP19 PR22 LAP18 C1orf215 CSDD1 LIN28 ZCCHC1 STMN1 STN1 stathmin |
| Molecular Weight(MW) | 19 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Stathmin 1 protein. |
| Entrez Gene | 3925 |
|---|---|
| Protein Accession | NP_001138926.1 |
Application
Western blot analysis of lysed extracts from 293 and HeLa cells using Stathmin 1 (Ab-25).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1 (Ab-25). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Stathmin 1 (Ab-25).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
