Application
Western blot analysis of STEAP1 in human spleen tissue lysate with STEAP1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of STEAP1 in human spleen tissue with STEAP1 antibody at 2.5 μg/mL.| Product Name | STEAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STEAP1 Antibody: STEAP, PRSS24, STEAP, Metalloreductase STEAP1, Six-transmembrane epithelial antigen of prostate 1 |
| Product description | STEAP1 Antibody: The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist.1) Hubert RS, Vivanco I, Chen E, et al. STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors. Proc. Natl. Acad. Sci. USA1999; 96:14523-8. |
| Immunogen | STEAP1 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STEAP1. The immunogen is located within the last 50 amino acids of STEAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| STEAP1 antibody can be used for detection of STEAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STEAP1 |
|---|---|
| Alternative Names | STEAP1 Antibody: STEAP PRSS24 STEAP Metalloreductase STEAP1 Six-transmembrane epithelial antigen of prostate 1 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | This STEAP1 antibody does not cross-react with other STEAP proteins. |
Application
Western blot analysis of STEAP1 in human spleen tissue lysate with STEAP1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of STEAP1 in human spleen tissue with STEAP1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | STEAP1 antibody can be used for detection of STEAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | STEAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | STEAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
