Application
Western blot analysis of STEAP2 in human prostate tissue lysate with STEAP2 antibody at 1 μg/mL.Application
Immunofluorescence of STEAP2 in Human Colon cells with STEAP2 antibody at 20 μg/mL.Application
Immunohistochemistry of STEAP2 in human colon tissue with STEAP2 antibody at 2.5 μg/mL.| Product Name | STEAP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STEAP2 Antibody: STMP, IPCA1, PUMPCn, STAMP1, PCANAP1, UNQ6507/PRO23203, Metalloreductase STEAP2, Prostate cancer-associated protein 1 |
| Product description | STEAP2 Antibody: The six-transmembrane epithelial antigen of prostate 2 (STEAP2) is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. Similar to two other members of the STEAP family (STEAP 3 and STEAP4), STEAP2 promotes both iron and copper reduction. STEAP2 expression in transfected cells also correlated with iron or copper uptake, suggesting that the STEAP family of proteins may function to stimulate iron and copper uptake. STEAP2 is widely expressed in many tissues in the plasma membrane, but is most highly expressed in prostate. At least three isoforms of STEAP2 are known to exist.1) Porkka KP, Helenius MA and Visakorpi T. Cloning and characterization of a novel six-transmembrane protein STEAP2, expressed in normal and malignant prostate. Lab Invest.2002; 82:1573-82. |
| Immunogen | STEAP2 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human STEAP2. The immunogen is located within the last 50 amino acids of STEAP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| STEAP2 antibody can be used for detection of STEAP2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STEAP2 |
|---|---|
| Alternative Names | STEAP2 Antibody: STMP IPCA1 PUMPCn STAMP1 PCANAP1 UNQ6507/PRO23203 Metalloreductase STEAP2 Prostate cancer-associated protein 1 |
| Molecular Weight(MW) | Predicted: 54 kDa Observed: 55 kDa |
| Tissue Specificity | This STEAP2 antibody does not cross-react with other STEAP proteins. |
Application
Western blot analysis of STEAP2 in human prostate tissue lysate with STEAP2 antibody at 1 μg/mL.
Application
Immunofluorescence of STEAP2 in Human Colon cells with STEAP2 antibody at 20 μg/mL.
Application
Immunohistochemistry of STEAP2 in human colon tissue with STEAP2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1312 - Human Prostate Tissue Lysate |
|---|---|
| Application Notes | STEAP2 antibody can be used for detection of STEAP2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | STEAP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | STEAP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
