Application
Western blot analysis of STEAP3 in HeLa cell lysate with STEAP3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of STEAP3 in Mouse Liver cells with STEAP3 antibody at 20 μg/mL.Application
Immunohistochemistry of STEAP3 in mouse liver tissue with STEAP3 antibody at 2.5 μg/mL.| Product Name | STEAP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STEAP3 Antibody: STMP3, TSAP6, AHMIO2, dudlin-2, Metalloreductase STEAP3, Dudulin-2, hTSAP6 |
| Product description | STEAP3 Antibody: The six-transmembrane epithelial antigen of prostate 3 (STEAP3) is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. Similar to two other members of the STEAP family (STEAP 2 and STEAP4), STEAP3 promotes both iron and copper reduction. STEAP3 is highly expressed in hematopoietic tissues and colocalizes with the transferrin endosome. Overexpression of STEAP3 stimulates iron reduction; mice lacking STEAP3 are deficient in erythroid ferrireductase activity, suggesting that STEAP3 is an endosomal ferrireductase required for transferrin-dependent iron uptake in erythroid cells.1) Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat. Genet.2005; 37:1264-9. |
| Immunogen | STEAP3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human STEAP3. The immunogen is located within the first 50 amino acids of STEAP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| STEAP3 antibody can be used for detection of STEAP3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STEAP3 |
|---|---|
| Alternative Names | STEAP3 Antibody: STMP3 TSAP6 AHMIO2 dudlin-2 Metalloreductase STEAP3 Dudulin-2 hTSAP6 |
| Molecular Weight(MW) | Predicted: 55 kDa Observed: 51 kDa |
| Tissue Specificity | This STEAP3 antibody does not cross-react with other STEAP proteins. |
Application
Western blot analysis of STEAP3 in HeLa cell lysate with STEAP3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of STEAP3 in Mouse Liver cells with STEAP3 antibody at 20 μg/mL.
Application
Immunohistochemistry of STEAP3 in mouse liver tissue with STEAP3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | STEAP3 antibody can be used for detection of STEAP3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | STEAP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | STEAP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
