Application
Western blot analysis in A431,A549 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with STIP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | STIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing protein, Hop, Renal carcinoma antigen NY-REN-11, Transformation-sensitive protein IEF SSP 3521, STIP1 |
| Product description | STIP1 mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).1) Onuoha,S.C., J. Mol. Biol. 379 (4), 732-744 (2008) Erlich,R.B., Glia 55 (16), 1690-1698 (2007) |
| Immunogen | This STIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-297 amino acids from the Central region of human STIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | STIP1 |
|---|---|
| Alternative Names | Stress-induced-phosphoprotein 1 STI1 Hsc70/Hsp90-organizing protein Hop Renal carcinoma antigen NY-REN-11 Transformation-sensitive protein IEF SSP 3521 STIP1 |
| Molecular Weight(MW) | 63 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A431,A549 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with STIP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
