Application
Western blot analysis of Stk39 in SK-N-SH cell lysate with Stk39 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of stk39 in human brain tissue with stk39 antibody at 20 μg/mL.Application
Immunohistochemistry of Stk39 in human brain tissue with Stk39 antibody at 2.5 μg/mL.| Product Name | Stk39 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Stk39 Antibody: DCHT, PASK, SPAK, DCHT, Ste-20-related kinase |
| Product description | Stk39 Antibody: The serine/threonine kinase Stk39 belongs to the STE20 family, a group of kinases that are known to interact with inflammation-related kinases (such as p38, JNK, NKCC1, PKC-theta, WNK and MLCK), and with transcription factor AP-1. The STE 20 family is involved in diverse biological phenomena, including cell differentiation, cell transformation/ proliferation, cytoskeleton rearrangement, and the regulation of ion transporters. STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain and is abundantly expressed in the brain. STK39 is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled co-transporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. Recent studies show that STK39 tend to be a novel candidate gene for autism and hypertension.1) Johnston AM, Nacelli G, Gonzales LJ, et al. SPAK, a STE20/SPS1-related kinase that activates the p38 pathway. Oncogene2000; 19:4290-7. |
| Immunogen | Stk39 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human Stk39. The immunogen is located within amino acids 450 - 500 of Stk39. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Stk39 antibody can be used for detection of stk39 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK39 |
|---|---|
| Alternative Names | Stk39 Antibody: DCHT PASK SPAK DCHT Ste-20-related kinase |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of Stk39 in SK-N-SH cell lysate with Stk39 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of stk39 in human brain tissue with stk39 antibody at 20 μg/mL.
Application
Immunohistochemistry of Stk39 in human brain tissue with Stk39 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | Stk39 antibody can be used for detection of stk39 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Stk39 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Stk39 Antibody is supplied in PBS containing 0.02% sodium azide. |
