Application
To detect hsTNF-receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human sTNF-receptor (XP-5276Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor.Application
To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.| Product Name | sTNF Receptor Type I Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FPF, MS5, p55, p60, TBP1, TNF-R, TNFAR, TNFR1, p55-R, CD120a, TNFR55, TNFR60, TNF-R-I, TNF-R55, TNFR1-d2, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1 |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF Receptor-I-I (human-soluble Tumor Necrosis Factor Receptor-I). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsTNF Receptor-I (0.3 μg/mL), a concentration of 0.9-1.1 μg/mL of this antibody is required. ELISA: To detect hsTNF Receptor-I by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsTNF Receptor-I. Sandwich: To detect hsTNF-receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sTNF-receptor (XP-5276Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor. Western Blot: To detect hsTNF Receptor-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF Receptor-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF1A |
|---|---|
| Alternative Names | FPF MS5 p55 p60 TBP1 TNF-R TNFAR TNFR1 p55-R CD120a TNFR55 TNFR60 TNF-R-I TNF-R55 TNFR1-d2 Tumor necrosis factor receptor superfamily member 1A Tumor necrosis factor receptor 1 TNF-R1 |
Application
To detect hsTNF-receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human sTNF-receptor (XP-5276Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor.
Application
To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsTNF Receptor-I (0.3 μg/mL), a concentration of 0.9-1.1 μg/mL of this antibody is required. ELISA: To detect hsTNF Receptor-I by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsTNF Receptor-I. Sandwich: To detect hsTNF-receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human sTNF-receptor (XP-5276Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor. Western Blot: To detect hsTNF Receptor-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF Receptor-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | sTNF Receptor Type I antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
