Application
Western blot analysis of sumo in HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunofluorescence of Sumo in HL60 cells with Sumo antibody at 20 μg/mL.Application
Immunocytochemistry of Sumo in HL60 cells with Sumo antibody at 5 μg/mL.| Product Name | Sumo Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Sumo Antibody: DAP1, GMP1, PIC1, SMT3, UBL1, OFC10, SENP2, SMT3C, SMT3H3, OK/SW-cl.43, Small ubiquitin-related modifier 1, GAP-modifying protein 1, SUMO-1 |
| Product description | Sumo Antibody: The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.1) Dohmen RJ. Sumo protein modification. Biochim. Biophys. Acta 2004; 1695:113-31. |
| Immunogen | Sumo antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human sumo. The immunogen is located within the first 50 amino acids of Sumo. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Sumo antibody can be used for detection of sumo by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SUMO1 |
|---|---|
| Alternative Names | Sumo Antibody: DAP1 GMP1 PIC1 SMT3 UBL1 OFC10 SENP2 SMT3C SMT3H3 OK/SW-cl.43 Small ubiquitin-related modifier 1 GAP-modifying protein 1 SUMO-1 |
| Sequence Similarities | 2) Cat. No. 17-009 - HL-60 Cell Slide |
Application
Western blot analysis of sumo in HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunofluorescence of Sumo in HL60 cells with Sumo antibody at 20 μg/mL.
Application
Immunocytochemistry of Sumo in HL60 cells with Sumo antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
|---|---|
| Application Notes | Sumo antibody can be used for detection of sumo by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Sumo antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Sumo Antibody is supplied in PBS containing 0.02% sodium azide. |
