Application
Western blot analysis of SUMO3 in mouse liver tissue lysate with SUMO3 antibody at (A) and (B) 2 μg/mL.Application
Immunofluorescence of SUMO3 in mouse liver tissue with SUMO3 antibody at 20 μg/mL.Application
Immunohistochemistry of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 5 μg/mL.| Product Name | SUMO3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SUMO3 Antibody: SMT3A, Smt3B, SMT3H1, SUMO-3, SMT3B, Small ubiquitin-related modifier 3, SMT3 homolog 1 |
| Product description | SUMO3 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.1) Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53. |
| Immunogen | SUMO3 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human SUMO3. The immunogen is located within the last 50 amino acids of SUMO3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SUMO3 antibody can be used for detection of SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SUMO3 |
|---|---|
| Alternative Names | SUMO3 Antibody: SMT3A Smt3B SMT3H1 SUMO-3 SMT3B Small ubiquitin-related modifier 3 SMT3 homolog 1 |
Application
Western blot analysis of SUMO3 in mouse liver tissue lysate with SUMO3 antibody at (A) and (B) 2 μg/mL.
Application
Immunofluorescence of SUMO3 in mouse liver tissue with SUMO3 antibody at 20 μg/mL.
Application
Immunohistochemistry of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | SUMO3 antibody can be used for detection of SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SUMO3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SUMO3 Antibody is supplied in PBS containing 0.02% sodium azide. |
